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Precipitation and Neutralization of Bacteriophage f2 by Rabbit Antibodies¹

From The Rockfeller University, New York, New York

Abstract

Summary and Conclusion: Neutralization and precipitation of bacteriophage f2 by G-immunoglobulin, 5S pepsinfragments and Fab' fragments were used to test the hypothesis that sterie hindrance is important in virus neutralization. There was no demonstrable difference in the capacity of intact G-immunoglobulin and 5S pepsin fragments to precipitate bacteriophage.The zones of equivalence with G-immunoglobulin and 5S pepsin fragments were broad. These results suggested that there was no differenee in the combining capacities of f2 for bivalent antibodies or for bivalent antibody fragments. As expected, the Fab' fragments did not precipitate with bacteriophage f2. The antibody to antigen ratios necessary to produce neutralization of bacteriophage correspond to points on the antibody excess end of the equivalence zone of the preeipitin curve. In all eases the kinetics of neutralization were first order. The G-immunoglobulin was most active and the Fab' fragment was least active. The 5S pepsin fragments were intermediate in activity. However, the neutralization constants were not strictly proportional to molecular weights. This suggests that virus neutralization may not be entirely explicable on the basis of steric hindrance.

Footnotes

1 These studies were supported by Grants AM 09428 and AI 06985 from the United States PublicHealth Service, and by Grant GB 3920 from the National Science Foundation.

² Present address: Department of Pathology,Duke University Medical Center, Durham, NorthCarolina.