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A Serologic Study of Herpesvirus Hominis Strains by Microneutralization Tests^1,2,

Department of Parasitology, School of Public Health, University of North Carolina, Chapel Hill, N. C., and the Respirovirus Unit, Virology Section, Laboratory Branch, Communicable Disease Center, Public Health Service, Department of Health, Education and Welfare, Atlanta, Georgia

Abstract

Twenty-four Herpes virus hominis strains, isolated from patients with differing clinical manifestations, were screened by macroneutralization tests ( procedure). The results suggested the presence of at least two serologic types. Nineteen (79%) appeared to be similar and were designated as type 1 strains. Five (21%) appeared to fall into a second group and were designated as type 2 strains. Detailed microquantal neutralization tests ( procedure) were performed with four strains and their respective antisera. Statistical analyses of the neutralizing potency (pN) and the normalized pN(NpN) values using the standard two-way analysis of variance followed by comparison of all means by Tukey's method supported the hypothesis of two distinct serologic types.

The microtechnique coupled with the quantal neutralization test exhibited a high degree of reproducibility and sensitivity. The microtest utilizes a minimum of materials, equipment and time as compared with the macroprocedures and may be performed in any virology laboratory with normal competency in tissue culture work. Calculation of pN values requires only a few mathematical steps. The recommended microtest ( procedure) provides the diagnostic virology laboratory with a statistically sound and practical procedure for the antigenic analysis of H. hominis isolates.

Footnotes

A portion of a dissertation submitted by the senior author to the University of North Carolina in partial fulfillment of the requirements for the degree of Doctor of Public Health in the School of Public Health.

Training was provided by the Laboratory Directors' Program, which is supported by Training Grant 5 T1 GM 567 from the National Institute of General Medical Sciences, National Institutes of Health, United States Public Health Service. The laboratory research was performed at the Laboratory Branch, Communicable Disease Center, Atlanta, Georgia, under the supervision of the junior author.

² Reprint request should be addressed to: Respirovirus Unit, Communicable Disease Center, Atlanta, Georgia 30333.

³ Present address: State Laboratory of Hygiene, 437 Henry Mall, Madison, Wisconsin 53706.

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