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The Direct Reaction Between Salmonella Enteritidis Endotoxin and Antibody

Measurement of 7S and 19S Antibody in Normal, Tolerant and Immune Sera¹

Department of Medicine, University of Wisconsin Medical School, Madison, Wisconsin

Abstract

Two preparations of Salmonella enteritidis endotoxin have been labeled with Cr⁵¹ and I¹²⁵. Using Farr's procedure of precipitation of antibody-bound antigen by ammonium sulfate, the direct reaction between endotoxin and antibody has been studied. I¹²⁵ labeling gave a product with greater specific activity and antibody binding. The binding of the labeled antigen by immune antibody was inhibited chiefly by Salmonella antigens of the same O antigenic specificity as S. enteritidis. Small amounts of what appeared to be "natural" 7S as well as 19S antibody were found in the serum of non-immunized persons and rabbits. This antibody was of broad specificity, cross-reacting with many Gram-negative and other antigens. The antibody of a rabbit made tolerant to the febrile effect of endotoxin was largely 19S, and of a rabbit immunized with whole organisms largely 7S. The serum of the tolerant rabbit protected mice from the lethal action of endotoxin, while the serum of the immune rabbit passively sensitized them.

Footnotes

Supported in part by USPHS Grant #AI-04412 and NIH Grant #OH-00044-06, and in part by a General Research Support Grant #144-5760-G220-3 to the University of Wisconsin Medical School from the National Institute of Health Division of Research Facilities and Resources.

Presented in part at the meetings of the American Association of Immunologists, Atlantic City, April, 1966.