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The Use of the Preparative Ultracentrifuge to Detect Primary Interactions between I¹³¹-Labeled Antigen and Antibody¹

Division of Allergy, Immunology and Rheumatology, Scripps Clinic and Research Foundation, La Jolla, California and the Department of Pediatrics, University of Chicago, Chicago, Illinois

Abstract

1. The different sedimentation characteristies of free I¹³¹-labeled antigen (I*antigen) and I*-antigen-antibody complexes in the preparative ultracentrifuge have been used as the basis for a test to detect and measure the primary interaction between antigen and antibody.

2. When serial dilutions of 10 different rabbit anti-bovine serum albumin sera were studied the results obtained with the ultracentrifuge method correlated well with data obtained by the ammonium sulfate technique.

3. The broad applicability of the ultracentrifuge method is illustrated by studies of the use of the method to detect antibody against bovine -globulin, and antigen difficult to study by most other primary binding techniques.

Footnotes

Supported by United States Public Health Service Research Grant AM-06894-01.

Presented in part to the American Association of Immunologists, March, 1959 (1).

² Supported in part by United States Public Health Service Grant 5-T1-AI-214 and in part by the Jennie L. Fay and Helen Fay Hunter trusts. Present address: Department of Pediatrics, University of Chicago, Chicago, Illinois 60637.