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Adoptive Cutaneous Anaphylaxis: a Technique for Studying Antibody Globulin Synthesis¹

From the Department of Microbiology and Immunology, Albert Einstein College of Medicine, Yeshiva University, New York, New York

Abstract

Lymph node, spleen and peritoneal exudate cells from immunized guinea pigs produce antibody when deposited in the skin of isologous recipients. Presence of antibody is revealed by injecting recipients intravenously with dye and specific antigen 2 to 7 days after cell deposition. No synthesis was detected in nonisogeneic guinea pigs sensitized against donor-type lymphoid cells or in certain other homologous, nonsensitized recipients.

Footnotes

¹ This investigation was supported by United States Public Health Service Grant AI-04131 from the National Institute of Allergy and Infectious Diseases.