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E-Globulin Antibody1
From the Children's Asthma Research Institute and Hospital, Denver, Colorado
Abstract
The skin-sensitizing activity in the sera of nine atopic patients, who are sensitive to ragweed pollen, and in a reagin-rich fraction of another patient's serum was precipitated by anti-E-globulin antiserum which did not contain any detectable antibody against G-, A-, M- or D-globulins. Absorption of the skin-sensitizing antibody was accompanied by the loss of E antibody in the supernatants. The skin-sensitizing activity in the patients' sera, determined by P-K reactions with antigen E, paralleled radioactivity of the E band which was observed by radioimmunodiffusion with the radioactively-labeled antigen, whereas no correlation was observed between the skin-sensitizing activity and either G or A antibodies. When one of the patients' sera was fractionated by chromatography on a DEAE cellulose column, gel filtration, sucrose density gradient ultracentrifugation and by zone electrophoresis on agarose gel, distributions of reaginic activity paralleled those of E antibody. These findings strongly support the hypothesis that reaginic antibodies in ragweed sensitive patients' sera are associated with E-globulin. Light chains of immunoglobulins were detected in E-globulin, indicating that E-globulin is a new immunoglobulin.
Footnotes
1 This work was supported mainly by research grants GB-4646 from the National Science Foundation and in part by Grant HE-05997 from the National Institutes of Health and United States Navy Contract Nonr-3656(12).
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