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The Use of a Water-Soluble Carbodiimide as a Coupling Reagent in the Passive Hemagglutination Test¹

From Milk and Food Research, Division of Environmental Engineering and Food Protection, United States Department of Health, Education, and Welfare, Public Health Service, Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio

Abstract

A simplified method is described for coupling proteins to red cells using the water-soluble carbodiimide, 1 - ethyl - 3 - (3 - dimethylaminopropyl) carbodiimide hydrochloride. The procedure is simpler than BDB coupling and the sensitized cells are more stable than those in the BDB system. The data indicated a marked similarity in the specificity and sensitivity of ECDI-and BDB-hemagglutination.

Footnotes

¹ This paper was presented in part at the annual meeting of the American Association of Immunologists, Atlantic City, New Jersey, April 11–16, 1966.