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M and G Antibodies in Mice: Dissociation of the Normal Immunoglobulin Sequence¹

From the Rockefeller University, New York, New York

Abstract

Methotrexate and colchicine administered during a primary response to S. adelaide in mice both preferentially inhibit G antibody formation. In these experiments, the M and G antibody responses seem to be independent of one another. The M antibody response is not prolonged by the immediate absence of a following G response. Also, the G antibody response is able to recover from the effects of both drugs and reach near-normal serum titers whether or not there is a preceding M response.

During a methotrexate inhibited response, normal levels of M can occur in the serum at a time when no pyroninophilic blast cells or secondary nodules are evident in the spleen. The recovery of G production is well correlated with the appearance of many hemocytoblasts in the splenic red pulp, although no secondary nodules or mature plasma cells can be found at this time.

A study has also been made of M and G antibody production in x-irradiated recipients after transfer of spleen cells from primed animals. When spleen cells are taken from immunized donors on day 2 or day 6 after injection of antigen, only M antibody can be demonstrated in the recipients. Spleen cells taken on day 8 or day 10 after antigen produce mostly G antibody in the recipients.

Footnotes

¹ This work is from a thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Rockefeller University. Presented in part at the Annual Meeting of the American Association of Immunologists at Chicago, April, 1964.

² Present address: Department of Bacteriology and Immunology, Harvard Medical School, Boston, Mass.