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C'1 Fixation by Human Isoagglutinins: Fixation of C'1 by G and M but Not by A Antibody¹

From the Children's Asthma Research Institute and Hospital, Denver, Colorado, and the Immunology Branch, National Cancer Institute, Bethesda, Maryland

Abstract

The C'1-fixing activity of human anti-A antibodies associated with three different immunoglobulins was studied 1) by measuring the amount of free C'1 left after the incubation of whole serum with red cell-antibody complexes and 2) by C'1a fixation and transfer test. The results indicated that both polymer and monomer forms of A-globulin antibody were incapable of fixing C'1 with the antigen. In the C'1a transfer test, it was found that the number of C'1a-fixing sites per cell was equal to the number of M antibody molecules combined to the cell, indicating that each M antibody molecule on a cell surface is capable of binding one molecule of C'1a, whereas at least two molecules of G antibody are required to generate a site capable of binding a molecule of C'1a.

Footnotes

¹ This work was supported partly by Research Grant AI-04985 from the United States Public Health Service.