HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Gerughty, R. M. Articles by Moon, H. D. Search for Related Content PubMed PubMed Citation Articles by Gerughty, R. M. Articles by Moon, H. D.

In vitro Transfer of Immunity by Ribosomes¹

From the Department of Pathology and the Cancer Research Institute, School of Medicine, University of California San Francisco Medical Center, San Francisco, California

Abstract

These studies were concerned with the transfer of immunologic activity to lymphoid cells of non-sensitized mice by incubation with ribosomal preparations obtained from splenic tissue of sensitized animals.

BALB/c mice were sensitized against homologous cells of C₃H mouse origin. Splenic lymphoid cells of these mice were able to lyse C₃H mouse cells in tissue culture. Ribosomes obtained from aliquots of the same spleens were incubated in vitro with lymphoid cells of nonsensitized BALB/c mice. After incubation with ribosomes, the lymphoid cells acquired the capacity to lyse C₃H target cells in vitro. The ability of the ribosomal preparation to transfer cytolytic activity was removed by prior treatment with ribonuclease. The ribosomal preparation had no direct lytic effect on the homologous target cells, but required incubation with viable lymphoid cells to demonstrate this effect. The antigenic dose level and the time between antigenic stimulus and harvesting of splenic tissue were examined with regard to the ability of the ribosomes to transfer cytolytic activity. Comparison of the cytolytic activity of lymphoid cells of sensitized animals with that of the in vitro "transformed" lymphoid cells revealed differences in both the rate and degree of the reaction.

Footnotes

¹ This investigation was supported by United States Public Health Service Research Grants CA-07191-03 and CA-03341-07 and Training Grant 2T1-GM 349-06.

² Present address: Department of Pathology, Medical College of South Carolina, Charleston, S. C. Requests for reprints should be addressed to Dr. Werner Rosenau in San Francisco.