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Hemolytic Activity of Lipoprotein-Depleted Serum and the Effect of Certain Anions on Complement¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California

Abstract

To study the role of serum lipoproteins in immune hemolysis, human serum was depleted of lipoproteins by centrifugation at a density of 1.21. The density was raised to this value by addition of NaBr. Treatment of serum with high concentrations of NaBr, KI or KSCN was found to inactivate C'3, C'4 and C'5. The same salts produced inactivation and changes in the physicochemical properties of purified C'3, C'4 and C'5. Lipoprotein-depleted serum was fully active in immune hemolysis after addition of purified C'3, C'4 and C'5. No lipid could be detected in the purified components. It is concluded that serum lipoproteins are not required for immune hemolysis by human complement.

Footnotes

¹ This is publication number 174 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California.

This work was supported by United States Public Health Service Grant 7007-01 and American Heart Association Grant 65-G166.

² Present address: Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires, Argentina.

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