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Immunoglobulin Production in Human Leukemia Cell Lines¹

From the Department of Biochemistry Research and the Department of Surgery, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York

Abstract

Production of immunoglobulins or immunoglobulin-like substances in cell culture lines of human leukemia and lymphoma has been demonstrated by radioiodine labeled antibody, fluorescent antibody and immunodiffusion techniques.

A direct reaction of a cell smear with radioiodine labeled antibody reagents specific for particular chains of immunoglobulin indicated that all the leukemia and lymphoma cell lines tested produce one or more classes of immunoglobulins.

Immunoglobulin-producing activity was shown to vary during culture with respect to the proportion of cells producing immunoglobulin. There is some evidence that synthesis of heavy and light chains proceeds at different rates in these cell lines.

Immunodiffusion studies of cell extracts revealed that the proteins produced by these cells were not distinguished antigenically with immunoglobulins of normal serum when tested with anti-sera specific to heavy and light chains.

Footnotes

¹ Supported in part by Grant AI-2342 from the National Institute of Allergy and Infectious Diseases and Grant CA-8204 from the National Cancer Institute, Bethedsda, Md., and Grant T-242 from the American Cancer Society. Presented at the Annual Meeting of the American Association of Immunologists at Atlantic City, N. J., April, 1966.

² On leave from the Institute of Cancer Immunopathology, Hokkaido University, Sapporo, Japan.

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