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Antibody Formation Initiated in Vitro

III. Antibody Formation and Allotypic Specificity Directed by Ribonucleic Acid from Peritoneal Exudate Cells¹

From the Public Health Research Institute of the City of New York, Inc., and the Department of Microbiology, College of Medicine, University of Illinois, Chicago, Illinois

Abstract

Studies on the initiation of antibody formation in vitro have been conducted with cells from rabbits homozygous at the b locus (A_b⁴/A_b⁴ and A_b⁵/A_b⁵) which determines antigenic markers on the light polypeptide chains that are common to the several classes of immunoglobulins of the rabbit. It has been found that the IgM antibody produced by lymph node (LN) fragments from one normal rabbit in response to ribonucleic acid extracted from antigenically stimulated peritoneal exudate (PE) cells of a second rabbit has allotypic determinants characteristic for the immune globulins of the donor of the PE cells and foreign to those of the donor of the LN cells. In contrast, the IgG antibody that is subsequently formed in the same culture has the light chain allotypic markers of the immune globulins of the donor of the lymph node cells.

Footnotes

¹ This work was supported by Grant AI 06899 and AI 07043 from the National Institutes of Health and by National Science Foundation Grant GB 4264.

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