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Inactivation and Density Studies with Para-Adenovirus 7 (SV40-Adenovirus "Hybrid" Population)¹

From the Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas

Abstract

Inactivation and density studies were performed with PARA-adenovirus 7, a "hybrid" population of defective SV40 and adenovirus. The ability of PARA and of "pure" SV40 to induce SV40 tumor antigen in simian cells were inactivated at similar slow rates upon exposure to ultraviolet light, while the infectivities of SV40 and PARA were rapidly destroyed. The infectivity of PARA was found to be more susceptible to inactivation by ultraviolet light than was the infectivity of either adenovirus 7 or SV40. The average 37% dose was 12 sec for PARA, 22 sec for adenovirus 7, and 28 sec for complete SV40. Both PARA and adenovirus 7 in the "hybrid" population were equally sensitive to photodynamic inactivation after incorporation of either neutral red or proflavine. Neither component was affected by treatment with ribonuclease or deoxyribonuclease. Both members showed similar rates of heat inactivation at 50°C. The PARA and adenovirus 7 in the "hybrid" stock both banded at a buoyant density of 1.34 in cesium chloride. Except for sensitivity to inactivation by ultraviolet light, PARA could not be differentiated from adenovirus.

Footnotes

¹ This investigation was supported in part by Public Health Service Grants CA-04600 and CA-10036 from the National Cancer Institute, and Grant AI-05382 from the National Institute for Allergy and Infectious Diseases, National Institutes of Health.

² American Cancer Society Professor of Virology