HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rossen, R. D. Articles by Butler, W. T. Search for Related Content PubMed Articles by Rossen, R. D. Articles by Butler, W. T.

The Sedimentation Behavior of Rhinovirus Neutralizing Activity in Nasal Secretion and Serum Following the Rhinovirus Common Cold

From the Department of Health, Education, and Welfare, United States Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Clinical Investigations, Bethesda, Maryland

Abstract

Nasal washings and serum from nine volunteers inoculated with rhinovirus NIH-1734 were fractionated by sucrose density gradient ultracentrifugation and the fractions were analyzed quantitatively for A-globulin, G-globulin and rhinovirus neutralizing activity.

Neutralizing activity in the nasal secretions was predominantly associated with 9S to 14S A-globulin whether the nasal secretion sample came from individuals developing antibody for the first time after experimental infection or from volunteers with naturally acquired antibody of long duration. In a few nasal secretion samples, 5S to 7S neutralizing activity was also found to be associated with both A-globulins and G-globulins and, in a single instance, nasal secretion contained 18S virus neutralizing activity which may have been associated with M-globulin.

Rhinovirus neutralizing activity in serum from all but one volunteer sedimented in the 5S to 7S region and was associated with fractions containing both A- and G-globulins. Sera from five subjects also contained virus neutralizing activity, which sedimented between 7S and 19S. Serum from one additional volunteer contained only 14S and 20S virus neutralizing activity. 19S antibody developed in only one of four volunteers infected for the first time with this strain of rhinovirus.

This article has been cited by other articles:

				R. M. Chanock Control of Acute Mycoplasmal and Viral Respiratory Tract Disease: The prospects of eventual successful immunoprophylaxis through vaccination are encouraging Science, July 17, 1970; 169(3942): 248 - 256. [PDF]