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Quantitative Studies on the Behavior of Sensitized Lymphoid Cells in Vitro

III. Conversion of "Normal" Lymphoid Cells to an Immunologically Active Status with RNA Derived from Isologous Lymphoid Tissues of Specifically Immunized Rats¹

From the Department of Medical Genetics, University of Pennsylvania School of Medicine, and the Wistar Institute, Philadelphia, Pennsylvania

Abstract

Incubation of otherwise normal, nonreactive rat lymphoid cells with crude preparations of RNA from specifically immunized isologous animals resulted in the acquisition of demonstrable destructive abilities by these cells against homologous target cells in culture. While the magnitude of the cytocidal capabilities of these treated lymphoid cells was low, it was immunologically specific—i.e., lymphoid cells incubated with RNA derived from animals immunized to a different antigen system proved to be ineffective in destroying homologous target cells. Addition of RNase drastically inhibited the immunologic conversion of lymphoid cells to an active status with immune RNA.

Footnotes

¹ This work was supported in part by United States Public Health Service Research Grant AI 07001.

² Fellow of the Helen Hay Whitney Foundation.

Present address: Department of Medical Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pa.

³ Present address: Institute für Virologie der Universität Würzburg, Würzburg, Germany.