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Density Separable Fractions during Growth of Measles Virus¹

From the Department of Pediatrics and Department of Bacteriology and Immunology, School of Medicine, State University of New York at Buffalo, Buffalo, New York

Abstract

The appearance of viral activities during the growth of measles virus in AV3 cells was analyzed employing density-gradient centrifugation in cesium chloride. Two density forms of infectious virus were demonstrated, with densities of 1.23 to 1.24 g/ml (M form) and 1.20 to 1.21 g/ml (L form). Sonication shifted the L form to a lower density, 1.16 g/ml (Ls form) with an increase in titer. The L form appeared early in the growth cycle, disappeared and was replaced by the M form which appeared later in the cycle. The M form was released and appeared to be the complete mature virion. The L form was poorly released and may represent either an aberrant end product or a precursor to the M form.

Hemagglutinin, complement-fixing and hemolytic activities accompanied the infectious peaks. Hemolytic activity corresponded closely to the infectivity peaks, but hemagglutinin and complement-fixing activity demonstrated a broad heterodensity. A high density (1.30 g/ml) noninfectious complement-fixing peak was shown.

Footnotes

¹ This work was supported in part by Research Grant AI-02396 from the National Institute of Allergy and Infectious Diseases.

² Present address: Department of Bacteriology, School of Medicine, Tohoku University; and National Hospital of Sendai, Sendai, Japan.

³ Recipient of a Research Career Award AI-K6-1136 from the National Institute of Allergy and Infectious Diseases.