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Isolation of Serum Fractions Capable of Producing Aleutian Disease in Mink¹

From the Department of Veterinary Pathology, Washington State University, Pullman, Washington, and the Department of Medicine, University of Minnesota, Minneapolis, Minnesota

Abstract

1. Serum protein fractionation of pooled mink serum containing the disease principle was used in an attempt to achieve some relative purification of the agent capable of producing Aleutian disease (AD) in mink.

2. All fractions of zone electrophoretic separations, , , and albumin as well as the three peaks of Sephadex G-200 separations, produced the disease when injected into normal mink.

3. Some relative purification of serum protein fractions capable of producing AD was achieved using gradient elution diethylaminoethyl (DEAE) column chromatography. Initial fractions did not include the infectious principle, whereas later fractions eluted with pH 5.5–5.0, 0.25–0.3 M phosphate buffers produced the disease in injected mink.

4. Rabbit antisera to DEAE fractions, known to contain infectivity for mink, showed no precipitating antibodies specific for AD serum when absorbed with normal mink serum.

Footnotes

¹ This study was supported in part by grants AM 07372, AI 06477 and 1-K3-AM 28231 from the United States Public Health Service and in part by a grant from the Minnesota Arthritis Foundation.

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				A. J. Kenyon, J. E. Gander, C. Lopez, and R. A. Good Isolation of Aleutian Mink Disease Virus by Affinity Chromatography Science, January 12, 1973; 179(4069): 187 - 189. [Abstract] [PDF]