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Inactivation of Poly-DL-Alanyl Bacteriophage T4 with Antisera Specific toward Poly-DL-Dalanine¹

From the Section of Chemical Immunology, The Weizmann Institute of Science, Rehovoth, Israel

Abstract

Bacteriophage T4B was reacted with N-carbody DL-alanine anhydride, leading to the covalent attachment of alanine residues to the phage, concomitant with extensive loss of viability. The alanylated survivors could be inactivated with anti-phage serum, but were also inactivated specifically with an antiserum possessing antipolyalanine specificity. By using the alanylated phage and the "decision" technique of Jerne and Avegno (14), concentrations of 10^-8 to 10^-9 g/ml of anti-polyalanine antibody could be detected.

Footnotes

¹ This investigation was supported in part by United States Public Health Service Research Grant AI-04715 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.

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