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Specificity of Fish Antibody

From the Life Sciences Research Division, IIT Research Institute, Chicago, Illinois

Abstract

Fish anti-BSA differed from rabbit anti-BSA in at least three ways. 1) The fish antibody had three equivalence points, 50, 25 and 6.25 mg/ml, whereas rabbit antibody had only one, 0.31 mg/ml. 2) The fish antibody had a larger amount of low-avid antibody. 3) The fish antibody migrated in the same direction but more slowly.

However, it could not be demonstrated that fish antibody has a higher cross-reactivity than rabbit antibody.

The modified agar gel diffusion method for demonstrating the equivalence point of an antigen-antibody system is recommended as a useful method. As little as 0.75 ml of either antibody or antigen is all that is required to determine the equivalence point.

This article has been cited by other articles:

				W. A. Janssen and C. D. Meyers Fish: Serologic Evidence of Infection with Human Pathogens Science, February 2, 1968; 159(3814): 547 - 548. [Abstract] [PDF]