| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
From the Children's Asthma Research Institute and Hospital, Denver, Colorado
Abstract
The reaginic activity in sera from ragweed-sensitive patients was inactivated after the precipitation of serum proteins with the rabbit antihuman globulin serum which did not contain any of the antibodies specific for 
G-, A-, M-globulins and for light chains of immunoglobulins. The rabbit antiserum gave a 1-globulin precipitin band with the reagin-containing fraction which contained A but none of the G-, M- or D-globulins. The specificity of the precipitin band was different from the A-anti-A band. By radioimmunoelectrophoresis and by radioimmunodiffusion, the antibody against ragweed pollen-extract (antigen E) was detected in the 1-globulin, indicating the presence of a unique 1-immunoglobulin. The protein was tentatively designated E-globulin. Precipitation of E-globulin anti-ragweed antibody in the reagincontaining fraction was accompanied by the loss of reaginic activity, whereas the precipitation of the A-globulin antibody in the fraction did not result in any decrease of reaginic activity. The results highly suggest that the reaginic activity is associated with E-globulin.
Footnotes
1 This work was supported by research grants AI-04985 from the United States Public Health Service and GB-4646 from the National Science Foundation.
This article has been cited by other articles:
|
|
 |
|
  R. R. Rich From Whence We Come (Happy Birthday to Us) J. Immunol., February 15, 2006; 176(4): 2031 - 2032. [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Sohn, S.-Y. Lee, K. E. Lee, and K.-E. Kim Comparison of VIDAS Stallertest and Pharmacia CAP Assays for Detection of Specific IgE Antibodies in Allergic Children Ann. Clin. Lab. Sci., January 1, 2005; 35(3): 318 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. McFadden Jr. A Century of Asthma Am. J. Respir. Crit. Care Med., August 1, 2004; 170(3): 215 - 221. [Full Text] [PDF]
|
|
|
|
|
|
Proceedings of the First Jack Pepys Occupational Asthma Symposium Am. J. Respir. Crit. Care Med., February 1, 2003; 167(3): 450 - 471. [Full Text] [PDF]
|
|
|
|
|
|
T. A. E. PLATTS-MILLS The Role of Immunoglobulin E in Allergy and Asthma Am. J. Respir. Crit. Care Med., October 15, 2001; 164(8): S1 - 5. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. POSTMA, G. H. KOPPELMAN, and D. A. MEYERS The Genetics of Atopy and Airway Hyperresponsiveness Am. J. Respir. Crit. Care Med., September 1, 2000; 162(3): S118 - 123. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Natter, S. Seiberler, P. Hufnagl, B. R. Binder, A. M. Hirschl, J. Ring, D. Abeck, T. Schmidt, P. Valent, and R. Valenta Isolation of cDNA clones coding for IgE autoantigens with serum IgE from atopic dermatitis patients FASEB J, November 1, 1998; 12(14): 1559 - 1569. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. J. Gleich and H. Kita Bronchial asthma: Lessons from murine models PNAS, March 18, 1997; 94(6): 2101 - 2102. [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gleich and G. Jacob Immunoglobulin E antibodies to pollen allergens account for high percentages of total immunoglobulin E protein Science, December 12, 1975; 190(4219): 1106 - 1108. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
