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Pathogenesis of Experimental Cholera: Identification of Choleragen (Procholeragen A)¹ by Disc Immunoelectro-Phoresis and Its Differentiation from Cholera Mucinase

From the Departments of Bacteriology and Mycology and Biochemistry, SEATO Medical Research Laboratory, Bangkok, Thailand²

Abstract

Choleragen, an antigenic, choleragenic moiety elaborated by some cholera vibrio strains in vitro, has been identified by disc immunoelectrophoresis as a protein migrating in a manner similar to human 7 S -globulin. Although preparations of choleragen have mucinolytic activity which is neutralized by antiserum against choleragen, the mucinase has been proven to be a separate entity which is not involved in the production of the diarrhea of experimental cholera. Similarly, it is choleragen, and not mucinase, which causes an increase of vascular permeability characterized by a delayed but sustained erythematous, edematous induration. Choleragen elaborated in vitro by an Ogawa serotype El Tor vibrio was serologically identical to that produced by the routinely used Inaba serotype classical cholera vibrio strain.

Footnotes

¹ Choleragen, as originally described (2), was considered to consist of two components, procholeragen A and procholeragen B, both of which were apparently required for the production of experimental cholera. With the observations (6) that procholeragen B is not required for some of the manifestations of the biologic activities of procholeragen A and that it can be replaced by a variety of buffer systems for the production of experimental cholera in orally challenged infant rabbits, it is proposed that the now unnecessary epithets, procholeragen A and procholeragen B, be discarded and the term, choleragen, be used to designate the nondialyzable, antigenic, choleragenic moiety. Such will be the practice in this article. Purified choleragen, therefore, is entirely equivalent to the "purified procholeragen A" of our previous reports (5, 6).

² Alternate address: APO, San Francisco 96346.

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