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A-Immunoglobulin from Rabbit Colostrum¹

From the Department of Microbiology, University of Florida, Gainesville, Florida

Abstract

An immunoglobulin was isolated from rabbit colostrum which differed from G-immunoglobulin in its -electrophoretic mobility, content of unique antigenic sites, higher sedimentation coefficient (10.8S) and higher carbohydrate content (3.2% hexose and 3.2% hexosamine). The colostral immunoglobulin appeared to be homologous with A-immunoglobulins of other species. A yield of 3 to 4.5 mg of A-immunoglobulin was obtained per milliliter of colostrum.

The rabbit A-immunoglobulin was used to prepare a specific anti- chain reagent in the goat. The serum concentration of A-immunoglobulin was found to be 55 to 217 µg/ml for various rabbits by quantitative precipitation with anti- chain.

The Ab4 allotypic sites controlled by the b locus were present on A-immunoglobulin but allotypic sites controlled by the a locus (Aa1 and Aa2) could not be identified on this protein.

Footnotes

¹ This investigation was supported by United States Public Health Service Research Grant AI-05042 from the National Institute of Allergy and Infectious Diseases.

² Recipient of Public Health Research Career Development Award K3-AI-11,469.

³ Recipient of Public Health Research Career Development Award 6-K3-HD-22.

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