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The Journal of Immunology, 1966, 97: 106-111.
Copyright © 1966 by The American Association of Immunologists, Inc.
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Immunologic Unresponsiveness in the Adult Guinea Pig

III. Variation of the Antigen and Vehicle of Suppression. Induction of Unresponsiveness in the Adult Rat1

Harold F. Dvorak2, Juanito B. Billote3, John S. McCarthy and Martin H. Flax

From the James Homer Wright Pathology Laboratories of the Massachusetts General Hospital, Boston, Massachusetts

Abstract

The induction of an antigen-specific state of immunologic unresponsiveness in the adult guinea pig has been extended to include several protein antigens other than serum proteins, with widely differing physical and antigenic properties. The extent of immunologic suppression varied with different antigens and could not be correlated with the molecular weight of the antigen. Single intravenous (i.v.) injections of human serum albumin (HSA), bovine {gamma}-globulin (BGG), ovalbumin, ferritin, hemocyanin and ribonuclease at the time of sensitization with the same antigen in complete Freund's adjuvant resulted in moderate to marked depression of the expected delayed skin test reactivity as well as a variable depression of antibody formation. Hemolytic antibody (7S {gamma}2 and macroglobulin) was completely suppressed with HSA, BGG, ribonuclease and ferritin but only incompletely with hemocyanin. Specific 7S {gamma}1 antibody (detected by PCA) was suppressed with all antigens tested except ribonuclease and hemocyanin. Animals receiving an i.v. injection of hemocyanin actually developed higher 7S {gamma}1 antibody titers than animals receiving only the sensitizing injections in adjuvant.

Suspending the "suppressing" antigen (HSA) in incomplete Freund's adjuvant led to a striking depression of delayed skin test reactivity and hemolytic antibody formation, whereas 7S {gamma}1 titers were identical with those of animals receiving either injection alone.

Delayed skin test reactions and hemolytic antibody titers were also strikingly depressed in the adult rat following a single intravenous injection of HSA in saline at the time of sensitization with the same antigen in complete Freund's adjuvant.

Footnotes

1 This work was supported by United States Public Health Service Grant 2TGM 19206 and by Career Development Award I-K3-AI 2040-01 to M. H. F.

2 Present address: Research Associate, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland.

3 Paul Dudley White Fellow of the Massachusetts Heart Association.




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M. Axelrad and D. A. Rowley
Hypersensitivity: Specific Immunologic Suppression of the Delayed Type
Science, June 28, 1968; 160(3835): 1465 - 1467.
[Abstract] [PDF]


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