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-Globulin and Antibody Formation in Vitro¹

V. The Secondary Response Made by Splenic White and Red Pulp with Reference to the Role of Secondary Nodules

From the Department of Pathology, New York University School of Medicine, New York, New York

Abstract

Separated white and red pulp of the spleen obtained from rabbits at various days after an intravenous injection of sheep erythrocytes (SE) were examined for numbers of plaque-forming cells (PFC) on SE-containing agar. It could be shown that both white and red pulp contained many antibody-forming cells on days 4 to 5, but that only the white pulp had the ability to develop PFC in vitro if taken on day 2 and kept in vitro for another 2 days.

With tissue taken on days 2 to 42 after a primary injection of SE, reexposure to SE in vitro and subsequent culturing for 4 to 5 days resulted in an increase in the numbers of PFC above initial levels. The white pulp usually contained a greater proportion of "sensitized" cells than the red, especially when kept in vitro for 1 day before the reexposure to antigen. Some white pulp preparations taken on days 4 to 12 reacted better when reexposed on day 1 than on day 0 of the culture period. Since this time period corresponds to the time of secondary nodule proliferation, the results are interpreted as indicating formation of "sensitized" cells in secondary nodules.

Footnotes

¹ These studies were supported by Grant AI-03076 from the United States Public Health Service.

² Fellow of the United States Public Health Service, 1-F-AI-14,689.

Present Address: Department of Immunochemistry, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C.

³ Recipient of Research Career Development Award GM-K3-15,222 from the United States Public Health Service.