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The Journal of Immunology, 1966, 96: 936-943.
Copyright © 1966 by The American Association of Immunologists, Inc.
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Fluorescent Antibody, Complement Fixation Method for Detection of Dengue Viruses in Mice1

R. W. Atchison, J. V. Ordonez2, G. E. Sather and W. McD. Hammon

From the Department of Epidemiology and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania

Abstract

The complement fixation fluorescent antibody (CF-FA) test was used for the detection of several types of dengue viruses, using serum of types 1 through 4, TH-36 and TH-Sman, in dilutions comparable to those of the conventional CF tests employed in this laboratory. Two-way cross-reaction experiments were done to determine the extent of CF-FA staining among dengues 1 and 3, Japanese B encephalitis, Murray Valley encephalitis and Eastern equine encephalomyelitis arboviruses and their respective antisera. Although cross-reactions could be observed among certain group B agents they did not cross with the representative of group A. It was also shown that the CF-FA test could detect inapparent dengue infection in two early (blind) passages of the unadapted dengue type 3 prototype strain.

Footnotes

1 This investigation was carried out under the sponsorship of the Commission on Viral Infections, Armed Forces Epidemiological Board. It was supported in part by the United States Army Medical Research and Development Command, Department of the Army, under Contract No. DA-49-193-MD-2042; in part as a special student problem by Dr. Ordonez, supported by Training Grant in Microbiology No. 5 T 1 AI 110 from the National Institutes of Health, United States Public Health Service; and in part by Research Grant No. AI 02686 from the National Institute of Allergy and Infectious Diseases.

2 Present address: Facultad de Sciencias Medicas, Department of Preventive and Social Medicine, Universitat of San Carlos of Guatemala, Guatemala City, Guatemala.






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