HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rapp, H. J. Articles by Borsos, T. Search for Related Content PubMed PubMed Citation Articles by Rapp, H. J. Articles by Borsos, T.

Forssman Antigen and Antibody: Preparation of Water Soluble Antigen and Measurement of Antibody Concentration by Precipitin Analysis, by C'1a Fixation and by Hemolytic Activity

From the Immunochemistry Section, Immunology Branch, National Cancer Institute, National Institutes of Health

Abstract

Purified fractions of rabbit antisera containing 19 S Forssman antibody were analyzed for antibody content on a molecular basis by precipitin analysis, by C'1a fixation and transfer and by direct hemolysis with complement. We have found that the C'1a fixation and transfer test detects all antibody molecules which react with water soluble Forssman antigen, while direct hemolytic assay detects about one third of these molecules. Possible reasons for this paradox are discussed.

Footnotes

¹ Part of this work was carried out at the Department of Microbiology, The Johns Hopkins University School of Hygiene and Public Health, under the direction of Dr. Manfred M. Mayer. It was supported by ONR Contract Nonr 248(60).