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Biosynthesis of Intrinsically Labeled Antibodies in Vitro¹

From the Cardiac Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, and the Department of Bacteriology and Immunology, Harvard Medical School

Abstract

1) A tissue culture method is described for the production of intrinsically labeled antibodies with specific radioactivities of 1 to 2 x 10⁷ cpm/mg antibody.

2) The kinetics of antibody production in this system were examined. It was found that large changes in the specific radioactivity of the antibodies occurred where the amount of isotopically labeled amino acid precursor in the culture medium was kept constant.

3) This variation in specific radioactivity was due to dilution of the labeled precursor by amino acid resulting from cell breakdown or protein release.

Footnotes

¹ Supported by Grants AI 04967 and HE 06664 of the National Institutes of Health and Grant 677 of the Greater Boston Chapter of the Massachusetts Heart Association.

² Advanced Research Fellow of the American Heart Association.