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The Journal of Immunology, 1966, 96: 1000-1005.
Copyright © 1966 by The American Association of Immunologists, Inc.

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Complement Fixation for Antigens on a Picogram Level1

Blake W. Moore2 and Vernon J. Perez

From the Department of Psychiatry, Washington University School of Medicine, St. Louis, Missouri

Abstract

1. A micro complement-fixation assay is described which is sensitive to 1 to 10 x 10-12 g of antigen.
2. The method was shown to be capable of measuring a specific antigen in brain extracts made from amounts of brain (dry weight) as small as 1.4 x 10-9 g, and in extracts from layers of cerebellum.

Footnotes

1 Supported by United States Public Health Service Research Grants CA-5228, NB-05270 and MH-04591, and Training Grant MH-7081.

2 Recipient of PHS Research Career Development Award 5-K3-GM-15, 543.







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