HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Spragg, J. Articles by Haber, E. Search for Related Content PubMed PubMed Citation Articles by Spragg, J. Articles by Haber, E. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB TRITIUM

Production of Antibody against Bradykinin: Demonstration of Specificity by Complement Fixation and Radio-Immunoassay¹

From the Department of Medicine, Harvard Medical School, and the Medical Services, Massachusetts General Hospital, Boston, Massachusetts

Abstract

1. The synthesis of a specifically oriented branch-chain copolymer antigen which was able to elicit the production of antibody directed against the polypeptide hormone, bradykinin, is reported.

2. The specificity of the anti-bradykinin antibody is determined in complement fixation studies and in radioactive binding studies.

3. A radio-immunoassay capable of detecting millimicrogram quantities of bradykinin is described. The specificity of the assay is demonstrated by the inability of the polypeptide hormones, vasopression and angiotensin II, to interfere with the binding of labeled bradykinin by specific anti-bradykinin antibody.

Footnotes

¹ A preliminary account of part of this study was presented at the International Symposium on Hypotensive Polypeptides, Florence, Italy, October 25–29, 1965. This investigation was supported by Grants AI-04967 and AI-04536 from the National Institute of Allergy and Infectious Diseases.

² Research Career Development Awardee, National Institute of Allergy and Infectious Diseases.

This article has been cited by other articles:

				M. Sela Antigenicity: Some Molecular Aspects Science, December 12, 1969; 166(3911): 1365 - 1374. [PDF]