HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Norman, L. Articles by Allain, D. S. Search for Related Content PubMed PubMed Citation Articles by Norman, L. Articles by Allain, D. S.

Preparation and Evaluation of Antigens for Use in the Serologic Diagnosis of Human Hydatid Disease

II. Isolation and Characterization from Extracts of Cysts of Echinococcus Multilocularis of Serologically Reactive Elements Found in Hydatid Fluid of Echinococcus Granulosus

From the Department of Health, Education, and Welfare, United States Public Health Service, Communicable Disease Center, Atlanta, Georgia

Abstract

1) Two of the parasite antigens identified in sheep hydatid fluid were demonstrated in extracts of Echinococcus multilocularis cysts. Serologic reactivity of the crude extract and fractions of the antigen appeared to be associated with the presence of at least one of these components.

2) Fractionation of Emc antigen by a column chromatographic method in which the antigen was cycled through a G-50 Sephadex column followed by a cycle through a long G-200 Sephadex column separated measurable amounts of nonspecific proteins, but did not "purify" the parasitic material. Gel-diffusion showed the most serologically reactive fraction (M_200A) to contain at least five of the nine components in the original crude extract.

3) The antigenic fraction M_200A was stable and reacted with the same degree of sensitivity and specificity as SHF antigen in the HA test for the diagnosis of hydatid disease.