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Comparisons of Antibodies Reacting with DNA

I. Systemic Lupus Erythematosus Sera and Rabbit Antibodies Induced by DNA-Methylated Bovine Serum Albumin Complexes¹

From the Department of Pharmacology, Tufts University School of Medicine, Boston, Massachusetts

Abstract

Six rabbit antisera induced by complexes of denatured deoxyribonucleic acid (DNA) and methylated bovine serum albumin (MBSA) were found to have C' fixation properties which differed from those of human (lupus erythematosus serum) anti-DNA antibodies. The rabbit anti-DNA-MBSA antibodies: 1) fixed C', with denatured DNA, more efficiently at 37°C than at 4°C; 2) were eluted from Sephadex G-200 columns in Fraction I as macroglobulins; and 3) were virtually completely inactivated by treatment with 0.1 M mercaptoethanol without subsequent alkylation. In these respects, lumpus erythematosus antibodies, in C' fixation reactions with DNA, behaved similarly to an anti-protein (anti-ribonuclease) system. They reacted more efficiently at 4°C than at 37°C; they were eluted from Sephadex G-200 columns in Fraction II as 7S globulins; and their C' fixation activity was only moderately reduced by 0.1 M mercaptoethanol. The lupus erythematosus sera also showed greater variability than the rabbit sera in the degree to which they reacted with native and denatured DNA.

Footnotes

¹ This work was performed under grant GB-2770 from the National Science Foundation.

² Postdoctoral Trainee in Biochemical Pharmacology (United States Public Health Service Graduate Training Grant 5T1 GM-765).