HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Levine, B. B. Articles by Levytska, V. Search for Related Content PubMed PubMed Citation Articles by Levine, B. B. Articles by Levytska, V.

Benzylpenicilloyl Specific Serum Antibodies to Penicillin in Man

I. Development of a Sensitive Hemagglutination Assay Method and Haptenic Specificities of Antibodies¹

From the Department of Medicine, New York University School of Medicine, New York, New York

Abstract

A convenient, reproducible, specific, and highly sensitive passive hemagglutination (HA) system for the detection of penicillin antibodies of benzylpenicilloyl (BPO) specificity in human and rabbit sera is described. Red blood cells were sensitized by coupling BPO groups to their surface membranes. The high sensitivity of the HA assay method is due to a) optimal sensitization of red blood cells by reaction with penicillin or benzylpenicillenic acid and b) the presence of dextran and normal serum in the serum diluent. The possible mechanisms whereby these factors increase the sensitivity of hemagglutination are discussed. The serum antibodies detected were shown to be specific mainly for the -diastereoisomeric BPO group, but to be specific also for other diastereoisomeric BPO groups. There was a heterogeneity among individual IgM human sera with regard to the stereospecificity of BPO-specific antibodies Because of its haptenic specificity and high sensitivity, this method may be useful in various types of immunologic studies.

Footnotes

¹ Supported by the Health Research Council of the City of New York under Contract U-1297.

² Career Investigator of the Health Research Council of the City of New York.

³ Dermatology Training Fellow under grant #T1 AM 5326 from the National Institute of Arthritis and Metabolic Diseases, United States Public Health Service.

This article has been cited by other articles:

				L. Olaison, L. Belin, H. Hogevik, and K. Alestig Incidence of {beta}-Lactam-Induced Delayed Hypersensitivity and Neutropenia During Treatment of Infective Endocarditis Arch Intern Med, March 22, 1999; 159(6): 607 - 615. [Abstract] [Full Text] [PDF]

				N. M. Vaz and B. B. Levine Immune Responses of Inbred Mice to Repeated Low Doses of Antigen: Relationship to Histocompatibility (H-2) Type Science, May 15, 1970; 168(3933): 852 - 854. [Abstract] [PDF]

				G. J. Gleich, R. C. Bieger, and R. Stankievic Antigen Combining Activity Associated with Immunoglobulin D Science, August 8, 1969; 165(3893): 606 - 606. [Abstract] [PDF]