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Antigenic Determinants of Human _1C- and _1G-Globulins¹

From the Children's Hospital Research Foundation and the Department of Pediatrics, University of Cincinnati College of Medicine

Abstract

Four distinct antigenic determinant groups have been found to be associated with _1C-globulin. Antigen A is present on _1A and antigen D is on a second breakdown product of _1C, designated _2D. Both are also present on _1C. Another antigen is also present on _2D which has been designated D_d but this is not present on _1C. A fourth antigen of _1C, designated B, is completely lost from the serum as _1C breaks down with aging. Antiserum containing antibody only to this group forms a _1C are with fresh serum but does not react with aged serum.

_2D loses most of its antigenicity on heating serum for 1 hr at 56°. _1A is unaffected by this treatment.

The breakdown of _1C occurring on reaction of fresh serum with zymosan or immune precipitates resembles in all respects that occurring with aging. Hydrazine or ammonia treatment, however, diminishes the mobility of _2D.

_1G, produced by reaction of isolated _1C with EAC'_1,4,2a cells, has A, D and D_d antigens but lacks the B antigen. Its mobility is similar to that of _1A.

The studies suggest that _1C undergoes marked unfolding and configurational changes as it breaks down. A close relationship may be assumed between the B antigen of _1C and the activity of the molecule in immune hemolytic reactions.

Footnotes

¹ This investigation was supported in part by grants AI-04472 and AI-03429 from the National Institute of Allergy and Infectious Diseases, United States Public Health Service, and by a grant from United Health Foundations, Inc.

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