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The Production of Antibodies to Mononucleotides, Oligonucleotides and DNA

From the Immunology Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri

Abstract

In the accompanying article we have described methods for preparing mono, oligo and polynucleotide protein conjugates (1). This article is concerned with the evaluation of the immunologic response to these preparations. We will present evidence for the formation of antibodies with specificity for nucleotides and DNA as demonstrated by precipitin and complement fixation reactions. The results of prelimiuary evaluation of the antibody response to tetrathymidylic acid in terms of the number of nncleotide residues involved in the antigenic site also will be reported.

Materials and Methods. Methods for preparing protein conjugates and tetrathymidylic acid are described in the preceding article (1).

Random bred albino rabbits (2.5 kg) were immunized with either 2 or 3 mg of nucleotide or polynucleotide-protein conjugate in complete Freund's adjuvant, distributed among the footpads. Antisera were obtained by cardiac puncture at 21 to 26 days. Globulin fractions were obtained by precipitation at 50% ammonium sulfate followed by dialysis.

Footnotes

¹ Postdoctoral Fellow of the United States Public Health Service, Grant 2 T1-AI-219.

² Recipient of a Research Career Development Award from the United States Public Health Service.

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