HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Halloran, M. J. Articles by Parker, C. W. Search for Related Content PubMed PubMed Citation Articles by Halloran, M. J. Articles by Parker, C. W.

The Preparation of Nucleotide-Protein Conjugates: Carbodiimides as Coupling Agents

From the Immunology Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri

Abstract

For some time it has been apparent that antibodies might be useful in the study of the fine structure of RNA and DNA. A major stumbling block has been the unavailability of a method which would render polynucleotides antigenic yet largely preserve their structural integrity. In approaching the problem of covalently conjugating polynucleotides to proteins as a means of stimulating antibody formation, we sought a procedure which would employ terminal nucleotide PO₄ or OH groups for coupling. Two ways in which the terminal PO₄ groups might be coupled covalently to protein would involve formation of a phosphodiester bond with protein seryl and threonyl residues or an N-P bond with protein -amino groups (Fig. 1, reactions 1 and 2 respectively). On the other hand, the OH group of a terminal sugar residue could react with protein carboxyl groups forming an ester (Fig. 1, reaction 3).

Footnotes

¹ Postdoctoral Fellow of the United States Public Health Service, Grant 2 T1-AI-219.

² Recipient of a Research Career Development Award from the United States Public Health Service.

This article has been cited by other articles:

				F. van Leeuwen, E. R. Wijsman, R. Kieft, G. A. van der Marel, J. H. van Boom, and P. Borst Localization of the modified base J in telomeric VSG gene expression sites of Trypanosoma brucei Genes & Dev., December 1, 1997; 11(23): 3232 - 3241. [Abstract] [Full Text] [PDF]