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Human Cytomegalovirus: Properties of the Complement-Fixing Antigen¹

From the World Health Organization International Reference Centre for Enteroviruses, Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas

Abstract

The correlation between infectivity, virus particle counts, and complement-fixing activity was studied with cell-associated and fluid phase preparations of two strains of human cytomegalovirus. CF antigen was detected only in cellassociated virus preparations and was not found in fluid phase virus preparations. The CF antigen obtained from cell-associated virus preparations was found to be stable at 4°C but was unstable at 37°C, which is just the reverse behavior of cytomegalovirus infectivity. When subjected to ultracentrifugation at speeds which sedimented virtually all of the virus particles, about 50% of the CF antigen behaved as a subviral "soluble" component and remained in the supernatant fluid.

Footnotes

¹ This study was supported in part by Research Grant CA-04600 from the National Cancer Institute and by Training Grant 5 T1 AI 74 and Research Grant AI 05382 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, United States Public Health Service.

² World Health Organization Senior Research Fellow.

Present address: Virus Research Department, Institute of Sera and Vaccines, Prague, Czechoslovakia.