The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1966, 96: 210-216.
Copyright © 1966 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karakawa, W. W.
Right arrow Articles by Krause, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karakawa, W. W.
Right arrow Articles by Krause, R. M.

The Production of Streptococcal Group-Specific Antibodies in Rabbits

II. A Fluorometric Method for Identification of Group A and A-Variant Agglutinins in Group A Antisera1

Walter W. Karakawa2 and Richard M. Krause

From the Department of Preventive Medicine, Washington University School of Medicine, St. Louis, Missouri

Abstract

A fluorometric method has been adapted to measure the relative concentrations of Groups A and A-variant antibodies in streptococcal Group A rabbit antisera. In this method FITC-labeled antibodies are adsorbed onto streptococcal cell walls and, after subsequent elution, measured in a fluorometer.

Inhibition of the reactions between Group A conjugate and homologous cell walls by Group A carbohydrate, and between Group A-variant conjugate and homologous cell walls by Group A-variant carbohydrate, confirmed the immunologic specificity of the two antibodies.

Footnotes

1 This study was supported in part by National Institutes of Health Research Grant 08027, National Heart Institute; National Institutes of Health Research Grant 06154, National Institute of Allergy and Infectious Diseases, and National Institutes of Health Training Grant 5T1 GM 807, National Institutes of General Medical Science.

It was conducted in part under the sponsorship of the Commission on Streptococcal and Staphylococcal Diseases, Armed Forces Epidemiological Board, and was supported by the Offices of the Surgeon General, Department of the Army, Washington, D. C.

2 National Institutes of Health Trainee in Epidemiology.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1966 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1966 by The American Association of Immunologists, Inc. All rights reserved.