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The Journal of Immunology, 1966, 96: 1-7.
Copyright © 1966 by The American Association of Immunologists, Inc.
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Mechanisms of {gamma}-Globulin Synthesis1

Saroj Tawde, Matthew D. Scharff2 and Jonathan W. Uhr

From the Irvington House Institute and Department of Medicine, New York University School of Medicine, and the Department of Cell Biology and Medicine, Albert Einstein College of Medicine, New York, New York

Abstract

Protein synthesis has been studied in lymph node cells obtained from rabbits hyperimmunized to bacteriophage T2. Provided ribonuclease activity was inhibited, almost all nascent protein synthesis occurred on polyribosomes. Pretreatment with 1 µg/ml actinomycin D, 7 hr prior to labeling, caused an 80% reduction of polyribosome-directed protein synthesis. Pulse-labeling studies of similar cell populations indicated that depression of RNA synthesis preceded the reduction of protein and {gamma}-globulin synthesis. When actinomycin D-treated cells were infected with Newcastle disease virus (an RNA virus), cells which had already ceased to make RNA and protein started to synthesize these macromolecules at rapid rates. Thus, the final steps of {gamma}-globulin synthesis by immune lymph node cells appear similar to those of protein synthesis in HeLa cells.

Footnotes

1 This investigation was supported by United States Public Health Service Research Grant No. AI-01821-08 and by the National Science Foundation Grant No. B-13118. It was done under the sponsorship of the Commission on Immunization of the Armed Forces Epidemiological Board and supported in part by the Office of The Surgeon General, Department of the Army.

2 Recipient of a United States Public Health Service Career Development Award.






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