HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ringle, D. A. Articles by Herndon, B. L. Search for Related Content PubMed PubMed Citation Articles by Ringle, D. A. Articles by Herndon, B. L.

A Micromanipulator-Based System for Immunologic Analyses with Microliter and Submicroliter Reactant Volumes

From the Biological Sciences Division, Midwest Research Institute, Kansas City, Missouri

Abstract

An efficient, micromanipulator-based test system is described for the immunologic analysis of microliter and submicroliter volumes by complement fixation, hemagglutination and immunodiffusion in both agar and cellulose acetate. With this system it is possible to carry out all of these tests on a total antiserum volume of about 1.5 µl. Antigen volume requirements for these tests are correspondingly low. In regard to reactant concentrations the sensitivity of the micromethods compares favorably with the sensitivity of the corresponding macromethods.