HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) A correction has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Pagano, J. S. Articles by Sedwick, W. D. Search for Related Content PubMed PubMed Citation Articles by Pagano, J. S. Articles by Sedwick, W. D.

The Specificity and Interaction with Poliovirus of an Inhibitory Bovine Serum¹

From The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania

Abstract

Bovine sera capable of inhibiting plaque formation of poliovirus may be highly selective in their action. A bovine serum that strongly inhibited plaque formation of the CHAT type 1 attenuated poliovirus had little effect on the 24 other type 1 strains tested, whether attenuated or virulent. An inhibitory factor, located in the 19 S serum globulin fraction by gel filtration with Sephadex G-200, did not affect replication of the sensitive virus, but it did initially neutralize virus in a cell-free system according to first order kinetics. The neutralized virus was reactivated at pH's below 5.0.

In correlating the inhibition in plaque reduction tests with the effects of cell-free neutralization, three results were observed: a) virus sensitive in both systems, b) virus nonsensitive in plaque reduction tests but sensitive to direct incubation with inhibitory serum and c) virus relatively nonsensitive in both systems.

In experiments with sequential neutralization, the addition of fresh bovine serum to mixtures containing virus previously exposed to bovine serum did not cause neutralization of the remaining infectious virus. However, type 1 antiserum did neutralize additional virus, although the amount neutralized and the rate of the second reaction were both somewhat diminished. Conversely, bovine serum added to complexes of virus and type 1 antiserum did not neutralize the residual virus.

Footnotes

¹ This work was supported in part by United States Public Health Service Grant AI-01799 and United States Public Health Service Training Grant TI-GM-142.

² Present address: Department of Medicine and Department of Bacteriology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina.