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Immunochemical and Genetic Studies of Two Distinct G-Immunoglobulins in BALB/c Mice¹

From the National Institutes of Health,³ Bethesda, Maryland

Abstract

Twenty-seven different immunoglobulins prepared from sera and urine of BALB/c mice with plasma cell tumors were tested with a variety of mouse allotype antisera reacting with immunoglobulins of normal BALB/c mice. None of the antisera obtained from 15 different donor-recipient combinations reacted in Ouchterlony precipitin tests with the one M- and three A-immunoglobulins nor with the ten and two light polypeptide chains from mice with plasma cell tumors. Several of the antisera gave weak precipitin reactions near the antigen well with some of the five immunoglobulins designated F; these reactions were interpreted as the probable result of contamination with small amounts of immunoglobulins from normal plasma cells.

The antisera distinguished between two classes of G-immunoglobulins designated G-Be1 and G-Be2. Some antisera gave dense precipitin bands with the three tumor-derived G-Be2 immunoglobulins as well as the corresponding G-Be2 immunoglobulin in normal serum but not with the three tumor-derived G-Be1 immunoglobulins. Similarly, some antisera gave dense precipitin bands with the three tumor-derived G-Be1 immunoglobulins as well as the corresponding G-Be1 immunoglobulin in normal serum but not with the three tumor-derived G-Be2 immunoglobulins. The allotypic specificities which distinguish between G-Be1 and G-Be2 (designated a1' and a1") were found to be associated with the papain Fc-fragment, a part of the heavy polypeptide chain.

Antisera specific for G-Be1 and G-Be2 (anti-a1' and anti-a1'') were used to test F₂ progeny for independent assortment or linkage. The a1' and a1'' allotypic specificities were either both present or both absent in the same F₂ progeny indicating close linkage in their genetic control.

Footnotes

¹ Presented in part at the annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City, New Jersey, April 10 to 14, 1965.

² Present address: Department of Microbiology, University of Illinois at the Medical Center, Chicago, Illinois 60612.

³ Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Laboratory of Biology, National Cancer Institute, and Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases.

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