HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Adler, F. L. Search for Related Content PubMed PubMed Citation Articles by Adler, F. L.

Studies on Mouse Antibodies

II. Mercaptoethanol-Sensitive 7 S Antibodies in Mouse Antisera to Protein Antigens¹

From the Division of Immunology, The Public Health Research Institute of the City of New York, Inc., New York, New York

Abstract

Mice injected once with ovalbumin or ferritin in Freund's adjuvant responded by producing 19 S and 7 S antibodies. Although 19 S antibody production continued for several months, the relative proportions of 7 S to 19 S changed from 5 to 1 at 2 weeks after immunization to 150 to 1 after 11 weeks. Sera that contained mainly 7 S antibody lost, upon reduction and alkylation, much of their aggregative efficiency and some of their precipitable antibody. The antibody in such reduced sera has been shown to combine promptly with antigen to form soluble complexes. That the effect of the reducing agent on aggregative properties was upon the antibody proper has been shown by experiments in which possible effects upon combining complement were shown to be irrelevant. It has also been shown that original activity was restored to treated serum by oxidation.

Reduced antisera against ferritin failed to agglutinate tanned cells coated with ferritin unless the concentration of ferritin used in coating of the cells was increased 10-fold above that needed to sensitize cells to agglutination by control sera. Complement fixation by reduced 7 S antibody has been shown to be diminished, especially when it was studied in dilute systems and at 37°C.

Footnotes

¹ This work was supported in part by Grant AI-01925 from the National Institute of Allergy and Infectious Diseases.