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Studies of Herpes Simplex Virus by the Gel Diffusion Technique

II. The Characterization of Viral and Soluble Precipitating Antigens¹

From the Children's Hospital of Philadelphia, Department of Pediatrics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

Abstract

Using gel diffusion technique, four precipitating antigens were found in preparations of herpes simplex virus. These antigens differed in their particle size, rate of diffusion in agar, density, chromatographic behavior, electrophoretic mobility and susceptibility to the destructive effects of various physical and chemical agents. All of these antigens elicited neutralizing antibodies when injected in laboratory animals.

Footnotes

¹ This investigation was supported by a research grant (AI-05612) from the National Institute of Allergy and Infectious Diseases, United States Public Health Service, Bethesda, Maryland, and by a supporting grant from the National Foundation, Inc., New York.