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Immunochemical Studies of Hemocyanin from the Giant Keyhole Limpet (Megathura Crenulata) and the Horseshoe Crab (Limulus Polyhemus)¹

From the California Institute of Technology, Pasadena, California Contribution No. 3221

Abstract

A method of obtaining a highly purified preparation of horseshoe crab hemocyanin which was found to be highly antigenic in rabbits is described.

The horseshoe crab hemocyanin migrated as a single band by cellulose acetate electrophoresis at pH 7.0 and 7.5 and showed a single line of precipitation with rabbit anti-HCH serum in agar-gel diffusion studies.

Cross-reactivity between the hemocyanins of the horseshoe crab and the giant keyhole limpet by quantitative precipitation, and by immunogenicity tests both in vitro and in vivo, were found to be negative.

Footnotes

¹ This study was supported by Grant AI-1355 from the National Institute of Allergy and Infectious Diseases, United States Department of Public Health, Bethesda, Maryland.

² Present Address. Oregon Regional Primate Research Center, 505 N. W. 185th Avenue, Beaverton, Oregon.

³ Present Address: Department of Microbiology, University of Queensland Medical School, Queensland, Australia.