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Observations on the Antibody Response of Rabbits to Mycobacterial Antigens¹

From the Department of Medicine, School of Medicine, Western Reserve University, Cleveland, Ohio

Abstract

The antibody response to soluble old tuberculin (OT), alum-precipitated purified protein derivative (PPD), OT incorporated in incomplete Freund's and whole killed mycobacteria in incomplete Freund's adjuvant has been studied in rabbits. Six of 12 animals injected with soluble OT and 3 of 8 given alum-precipitated PPD produced antibody measurable by hemagglutination techniques. This antibody was of short duration and was of the _1M type exclusively. Five of 8 animals responded to OT in adjuvant and produced antibodies of both _1M and 7 S-₂ types. All animals responded to immunization with whole organisms in adjuvant. In these latter animals a transition from an early production of both classes of antibody, with _1M antibody predominating in the earliest positive sera, to persistent exclusive synthesis of 7 S-₂-immunoglobulin was observed. Following a second challenge an anamnestic response was not observed in any of the animals. That antibody which was synthesized following reinjection without adjuvant was of the _1M type and was not accelerated in its appearance, suggesting a second primary rather than a secondary event. The data are in accord with the concept that the evocation of the 7 S-₂ response requires a more intense stimulus than does the _1M response. The production of 7 S-₂ antibody, however, does not necessarily indicate preparation of the animal for a subsequent anamnestic response.

Footnotes

¹ Supported by a grant from the Indiana Tuberculosis Association. A preliminary report of part of this work was presented at the Annual Meeting of the American Thoracic Society in New York City on May 25, 1964.