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Electrophoretic and Immunoelectrophoretic Characterization of Soluble Cellular Proteins in Rat Liver Cell Sap

From the Department of Gastroenterology, Walter Reed Army Institute of Research, Washington, D. C.

Abstract

Soluble protein composition of the cytoplasmic sap of the hepatic cells in rats was analyzed by electrophoretic and immunoelectrophoretic techniques. The electrophoretic pattern of hepatic cytoplasmic sap at a particular time of day was relatively constant if daily activity of rats was regulated. The majority of cytoplasmic sap proteins in rat liver differed from serum proteins in electrophoretic and immunoelectrophoretic properties. Cytoplasmic sap proteins proper could be grouped into at least six components having discrete electrophoretic and antigenic properties. From the findings, it is suggested that intracellular colloidal osmotic pressure is maintained primarily by proteins that differ from those found in body fluid. Almost complete absence of serum proteins in the hepatic cells also indicates that serum proteins leave cells rapidly after synthesis.