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Immunohemolysis as a Method for Quantitation of the A Antigen of Human Erythrocytes¹

From the Department of Human Genetics, The University of Michigan Medical School, Ann Arbor, Michigan

Abstract

A hemolytic system has been developed for the study of the strength of the human A erythrocyte antigen. Under identical test conditions cell suspensions standardized colorimetrically have been incubated with a constant amount of reagent and C'. The percentage of hemolysis obtained is taken as a measure of strength for the A antigen. The method is highly reproducible and reveals quantitative differences between and within the A subtypes. Results are also presented and discussed from studies on effects of concentrations of red cells, reagent, and C', as well as on the interrelation between reagent and C'. Since the requirements of the method are relatively small with regard to equipment and consumption of reagent, it may have additional applicability in quantitative studies on hemolytic antibody, C' or red cell antigens.

Footnotes

¹ This work was supported in part by AEC Contract AT(11-1)405 while the author was on a National Institutes of Health postdoctoral fellowship (GPD-15, 166).