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An Immunologic Study of the "Lipovirus"¹

From the Department of Microbiology, Harvard School of Public Health, Boston, Massachusetts

Abstract

Sheep erythrocytes treated with lipovirus were so altered that they became agglutinated in the presence of specific antiserum. The serum agglutinating factor was stable to heat at 56°C for 30 min and was absorbed by lipovirus-treated red cells. This agglutination was specifically inhibited by pretreatment of antiserum with lipovirus. Rabbits injected with lipovirus responded with the formation of antibody in high titers as demonstrated by this reaction. Serums from 28 rabbits injected with a variety of viral, rickettsial, and bacterial agents and cell components did not agglutinate lipovirus-sensitized erythrocytes.

Complement-fixing antigen specific for the lipovirus was prepared by concentrating the specific involved shrunken cells. Antibody responses were demonstrated in all rabbits injected with the lipovirus but not in those injected with uninfected cells. Some animals immunized with lipovirus grown in human cells showed rises in antibody against uninfected chick cell antigen. Similarly, some injected with lipovirus in chick cells showed rises in antibody against human cells.

A variety of antisera or paired acute and convalescent sera were tested for "lipovirus" antibody. There is no evidence that the lipovirus is immunologically related to any of the following viruses: vaccinia, herpes simplex, varicella, cytomegalo-, adeno-, SV₄₀, polyoma, influenza types A and B, para-influenza types 1, 2, 3 and 4, measles, mumps, respiratory syncytial and Newcastle disease viruses.

Footnotes

¹ Supported by Research Grants GM-K3-15, 131 and AI04998 from the National Institutes of Health.

This article has been cited by other articles:

				J. B. Little and R. S. Chang Radiation Resistance in Lipovirus-Altered Human Cells Science, June 25, 1965; 148(3678): 1746 - 1747. [Abstract] [PDF]