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Multiplicity of Antibody Proteins in Rabbit Anti-p-Azobenzenearsonate Sera¹

From Department of Biochemistry Research, Roswell Park Memorial Institute, New York State Department of Health, Buffalo 3, New York

Abstract

Rabbit antibodies against the p-azobenzenearsonate (R_p) group were studied with respect to their immunoelectrophoretic and chromatographic properties as well as their molecular size. Anti-R_p antibody activity was found in three different protein components of the antisera by the use of radioimmunoelectrophoresis with I¹³¹-R_p-insulin as a test antigen. The three antibody components, although all were directed against the same determinant group, differed in their reaction with horse antiserum against rabbit globulin. They were partially separated by gel filtration with Sephadex G-200 according to their molecular size and by chromatography on diethylaminoethyl cellulose. The antibodies of three different protein types were identified as the -type (6 S), the ₂-type (16 S) and the ₁-type (probably 9 S and 6 S).

The -type contains -globulins of a wide range of electrophoretic mobility, but can be fractionated by chromatography into two distinct fractions which differ in their mobility, ₂ and ₁. It was present in all the antisera tested and carried a major part of the antibody activity.

The ₂-type appears to be the same as the ₂ or ₁maeroglobulin previously found in rabbit serum. It was detected in most of the antisera tested.

The ₁-type is probably associated with the 9 S component and possibly also with the 6 S component. It seems to be the counterpart of _2A-globulin in the human. It was detected in only two of the six individual antisera tested.

Footnotes

¹ Supported by Grant AI 05158 from National Institute of Allergy and Infectious Diseases, United States Public Health Service.

² Fulbright Scholar on leave from Department of Biochemistry, Kyushu University School of Medicine, Fukuoka, Japan.

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